Abstract
The antioxidant effect of extracts from leaves, raw cortex, and roasted cortex of Du-zhong was evaluated using various lipid peroxidation models. The inhibitory activity of extracts of Du-zhong (200 μg/mL) on the peroxidation of linoleic acid measured by thiocyanate method followed the order leaves (99.9%) > roasted cortex (95.9%) > raw cortex (77.2%) at 60 h of incubation. The IC20 for leaves, roasted cortex, and raw cortex on the peroxidation of liposome, induced by Fe3+/H2O2/ascorbic acid, was <0.06, 0.24, and 0.81 mg/mL, respectively. The thiobarbituric acid reactive substances values for leaves, roasted cortex, and raw cortex were 0.12, 1.54, and 1.81 μmol of malondialdehyde/ mg of protein in enzyme-mediated microsomal peroxidation and 0.08, 0.69, and 0.88 μmol of malondialdehyde/mg of protein in the nonenzyme-mediated microsomal peroxidation, respectively. The antioxidant activity of extracts of Du-zhong correlates to their polyphenol content. The results presented herein indicate that extracts of Du-zhong leaves may be useful in inhibiting membrane lipid peroxidation and preventing free radical-linked disease.
| Original language | English |
|---|---|
| Pages (from-to) | 3952-3957 |
| Number of pages | 6 |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume | 46 |
| Issue number | 10 |
| Publication status | Published - 1998 Oct 1 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
- Agricultural and Biological Sciences(all)
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Antioxidant Activity of Extracts from Du-zhong (Eucommia ulmoides) toward Various Lipid Peroxidation Models in Vitro. / Yen, Gow Chin; Hsieh, Chiu Luan.
In: Journal of Agricultural and Food Chemistry, Vol. 46, No. 10, 01.10.1998, p. 3952-3957.Research output: Contribution to journal › Article
TY - JOUR
T1 - Antioxidant Activity of Extracts from Du-zhong (Eucommia ulmoides) toward Various Lipid Peroxidation Models in Vitro
AU - Yen, Gow Chin
AU - Hsieh, Chiu Luan
PY - 1998/10/1
Y1 - 1998/10/1
N2 - The antioxidant effect of extracts from leaves, raw cortex, and roasted cortex of Du-zhong was evaluated using various lipid peroxidation models. The inhibitory activity of extracts of Du-zhong (200 μg/mL) on the peroxidation of linoleic acid measured by thiocyanate method followed the order leaves (99.9%) > roasted cortex (95.9%) > raw cortex (77.2%) at 60 h of incubation. The IC20 for leaves, roasted cortex, and raw cortex on the peroxidation of liposome, induced by Fe3+/H2O2/ascorbic acid, was <0.06, 0.24, and 0.81 mg/mL, respectively. The thiobarbituric acid reactive substances values for leaves, roasted cortex, and raw cortex were 0.12, 1.54, and 1.81 μmol of malondialdehyde/ mg of protein in enzyme-mediated microsomal peroxidation and 0.08, 0.69, and 0.88 μmol of malondialdehyde/mg of protein in the nonenzyme-mediated microsomal peroxidation, respectively. The antioxidant activity of extracts of Du-zhong correlates to their polyphenol content. The results presented herein indicate that extracts of Du-zhong leaves may be useful in inhibiting membrane lipid peroxidation and preventing free radical-linked disease.
AB - The antioxidant effect of extracts from leaves, raw cortex, and roasted cortex of Du-zhong was evaluated using various lipid peroxidation models. The inhibitory activity of extracts of Du-zhong (200 μg/mL) on the peroxidation of linoleic acid measured by thiocyanate method followed the order leaves (99.9%) > roasted cortex (95.9%) > raw cortex (77.2%) at 60 h of incubation. The IC20 for leaves, roasted cortex, and raw cortex on the peroxidation of liposome, induced by Fe3+/H2O2/ascorbic acid, was <0.06, 0.24, and 0.81 mg/mL, respectively. The thiobarbituric acid reactive substances values for leaves, roasted cortex, and raw cortex were 0.12, 1.54, and 1.81 μmol of malondialdehyde/ mg of protein in enzyme-mediated microsomal peroxidation and 0.08, 0.69, and 0.88 μmol of malondialdehyde/mg of protein in the nonenzyme-mediated microsomal peroxidation, respectively. The antioxidant activity of extracts of Du-zhong correlates to their polyphenol content. The results presented herein indicate that extracts of Du-zhong leaves may be useful in inhibiting membrane lipid peroxidation and preventing free radical-linked disease.
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UR - http://www.scopus.com/inward/citedby.url?scp=0001075828&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0001075828
VL - 46
SP - 3952
EP - 3957
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 10
ER -