Antioxidant Activity of Extracts from Du-zhong (Eucommia ulmoides) toward Various Lipid Peroxidation Models in Vitro

Gow Chin Yen, Chiu Luan Hsieh

Research output: Contribution to journalArticle

243 Citations (Scopus)

Abstract

The antioxidant effect of extracts from leaves, raw cortex, and roasted cortex of Du-zhong was evaluated using various lipid peroxidation models. The inhibitory activity of extracts of Du-zhong (200 μg/mL) on the peroxidation of linoleic acid measured by thiocyanate method followed the order leaves (99.9%) > roasted cortex (95.9%) > raw cortex (77.2%) at 60 h of incubation. The IC20 for leaves, roasted cortex, and raw cortex on the peroxidation of liposome, induced by Fe3+/H2O2/ascorbic acid, was <0.06, 0.24, and 0.81 mg/mL, respectively. The thiobarbituric acid reactive substances values for leaves, roasted cortex, and raw cortex were 0.12, 1.54, and 1.81 μmol of malondialdehyde/ mg of protein in enzyme-mediated microsomal peroxidation and 0.08, 0.69, and 0.88 μmol of malondialdehyde/mg of protein in the nonenzyme-mediated microsomal peroxidation, respectively. The antioxidant activity of extracts of Du-zhong correlates to their polyphenol content. The results presented herein indicate that extracts of Du-zhong leaves may be useful in inhibiting membrane lipid peroxidation and preventing free radical-linked disease.

Original languageEnglish
Pages (from-to)3952-3957
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume46
Issue number10
Publication statusPublished - 1998 Oct 1

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Eucommiaceae
Eucommia ulmoides
Malondialdehyde
Lipid Peroxidation
cortex
lipid peroxidation
Antioxidants
antioxidant activity
Lipids
Thiobarbituric Acid Reactive Substances
peroxidation
extracts
Polyphenols
Linoleic Acid
Membrane Lipids
Liposomes
Ascorbic Acid
Free Radicals
Proteins
leaves

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

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title = "Antioxidant Activity of Extracts from Du-zhong (Eucommia ulmoides) toward Various Lipid Peroxidation Models in Vitro",
abstract = "The antioxidant effect of extracts from leaves, raw cortex, and roasted cortex of Du-zhong was evaluated using various lipid peroxidation models. The inhibitory activity of extracts of Du-zhong (200 μg/mL) on the peroxidation of linoleic acid measured by thiocyanate method followed the order leaves (99.9{\%}) > roasted cortex (95.9{\%}) > raw cortex (77.2{\%}) at 60 h of incubation. The IC20 for leaves, roasted cortex, and raw cortex on the peroxidation of liposome, induced by Fe3+/H2O2/ascorbic acid, was <0.06, 0.24, and 0.81 mg/mL, respectively. The thiobarbituric acid reactive substances values for leaves, roasted cortex, and raw cortex were 0.12, 1.54, and 1.81 μmol of malondialdehyde/ mg of protein in enzyme-mediated microsomal peroxidation and 0.08, 0.69, and 0.88 μmol of malondialdehyde/mg of protein in the nonenzyme-mediated microsomal peroxidation, respectively. The antioxidant activity of extracts of Du-zhong correlates to their polyphenol content. The results presented herein indicate that extracts of Du-zhong leaves may be useful in inhibiting membrane lipid peroxidation and preventing free radical-linked disease.",
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Antioxidant Activity of Extracts from Du-zhong (Eucommia ulmoides) toward Various Lipid Peroxidation Models in Vitro. / Yen, Gow Chin; Hsieh, Chiu Luan.

In: Journal of Agricultural and Food Chemistry, Vol. 46, No. 10, 01.10.1998, p. 3952-3957.

Research output: Contribution to journalArticle

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