Anticancer activity of rhamnoallosan against DU-145 cells is kinetically complementary to coexisting polyphenolics in psidium guajava budding leaves

Kuan Chou Chen, Chiu-Lan Hsieh, Kuan Dar Huang, Yaw Bee Ker, Charng Cherng Chyau, Robert Y. Peng

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Psldlum guajava L. is a valuable farm fruit plant having many medicinal uses. Previously its budding leaves (PE) were shown to contain huge amounts of soluble polyphenols (SP) including (in mg/g) gallic acid (348), catechin (102), epicatechin (60), rutin (100), quercetin (102), and rutin (100) and to exhibit potent anticancer activity. However, reconstitution of these polyphenols recovered only 40% of the original bioactivity, and the soluble carbohydrate (SC) portion in PE was suspected to contribute the remaining. PE contained a novel rhamnoallosan, which had a carbohydrate/protein (w/w) ratio = 29.06%/10.27% (=2.83, average molecular mass of 5029 kDa), characteristically evidencing a peptidoglycan, consisting of a composition (mole % ratio) of rhamnose/allose/arabinose/tallose/ xylose/fucose/glucose/mannose/galactose = 36.05:24.24:8.76:7.95:7.37:5.90:3.69:3.19:2.85 and of amino acid (in wt %) glycine/leucine/proline/alanine/methionine/isoleucine/valine/histidine/tyrosine/ phenylalanine/cysteine/aspartic acid/lysine/glutamic acid=37.12:12.68:10.05:8. 97:5.99:4.89:4.83:4.25: 4.05:2.78:1.86:1.10:0.73:0.70. Kinetic analysis showed comparable apparent cell-killing rate coefficients (K app) to be 4.03 x 10 3 and 2.92 x 10 3 cells mg -1 h -1 respectively, by SP and SC, evidencing the complementary anti-DU-145 bioactivity in nature.

Original languageEnglish
Pages (from-to)6114-6122
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume57
Issue number14
DOIs
Publication statusPublished - 2009 Jul 22

Fingerprint

Psidium
Psidium guajava
Polyphenols
Rutin
polyphenols
Ala-Leu-Pro-Met
Catechin
Carbohydrates
rutin
Bioactivity
carbohydrates
allose
leaves
Rhamnose
Gallic Acid
Arabinose
Fucose
fucose
Peptidoglycan
Isoleucine

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

@article{d1d9b0b5ccd542e8878bc4129deb45b4,
title = "Anticancer activity of rhamnoallosan against DU-145 cells is kinetically complementary to coexisting polyphenolics in psidium guajava budding leaves",
abstract = "Psldlum guajava L. is a valuable farm fruit plant having many medicinal uses. Previously its budding leaves (PE) were shown to contain huge amounts of soluble polyphenols (SP) including (in mg/g) gallic acid (348), catechin (102), epicatechin (60), rutin (100), quercetin (102), and rutin (100) and to exhibit potent anticancer activity. However, reconstitution of these polyphenols recovered only 40{\%} of the original bioactivity, and the soluble carbohydrate (SC) portion in PE was suspected to contribute the remaining. PE contained a novel rhamnoallosan, which had a carbohydrate/protein (w/w) ratio = 29.06{\%}/10.27{\%} (=2.83, average molecular mass of 5029 kDa), characteristically evidencing a peptidoglycan, consisting of a composition (mole {\%} ratio) of rhamnose/allose/arabinose/tallose/ xylose/fucose/glucose/mannose/galactose = 36.05:24.24:8.76:7.95:7.37:5.90:3.69:3.19:2.85 and of amino acid (in wt {\%}) glycine/leucine/proline/alanine/methionine/isoleucine/valine/histidine/tyrosine/ phenylalanine/cysteine/aspartic acid/lysine/glutamic acid=37.12:12.68:10.05:8. 97:5.99:4.89:4.83:4.25: 4.05:2.78:1.86:1.10:0.73:0.70. Kinetic analysis showed comparable apparent cell-killing rate coefficients (K app) to be 4.03 x 10 3 and 2.92 x 10 3 cells mg -1 h -1 respectively, by SP and SC, evidencing the complementary anti-DU-145 bioactivity in nature.",
author = "Chen, {Kuan Chou} and Chiu-Lan Hsieh and Huang, {Kuan Dar} and Ker, {Yaw Bee} and Chyau, {Charng Cherng} and Peng, {Robert Y.}",
year = "2009",
month = "7",
day = "22",
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Anticancer activity of rhamnoallosan against DU-145 cells is kinetically complementary to coexisting polyphenolics in psidium guajava budding leaves. / Chen, Kuan Chou; Hsieh, Chiu-Lan; Huang, Kuan Dar; Ker, Yaw Bee; Chyau, Charng Cherng; Peng, Robert Y.

In: Journal of Agricultural and Food Chemistry, Vol. 57, No. 14, 22.07.2009, p. 6114-6122.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Anticancer activity of rhamnoallosan against DU-145 cells is kinetically complementary to coexisting polyphenolics in psidium guajava budding leaves

AU - Chen, Kuan Chou

AU - Hsieh, Chiu-Lan

AU - Huang, Kuan Dar

AU - Ker, Yaw Bee

AU - Chyau, Charng Cherng

AU - Peng, Robert Y.

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AB - Psldlum guajava L. is a valuable farm fruit plant having many medicinal uses. Previously its budding leaves (PE) were shown to contain huge amounts of soluble polyphenols (SP) including (in mg/g) gallic acid (348), catechin (102), epicatechin (60), rutin (100), quercetin (102), and rutin (100) and to exhibit potent anticancer activity. However, reconstitution of these polyphenols recovered only 40% of the original bioactivity, and the soluble carbohydrate (SC) portion in PE was suspected to contribute the remaining. PE contained a novel rhamnoallosan, which had a carbohydrate/protein (w/w) ratio = 29.06%/10.27% (=2.83, average molecular mass of 5029 kDa), characteristically evidencing a peptidoglycan, consisting of a composition (mole % ratio) of rhamnose/allose/arabinose/tallose/ xylose/fucose/glucose/mannose/galactose = 36.05:24.24:8.76:7.95:7.37:5.90:3.69:3.19:2.85 and of amino acid (in wt %) glycine/leucine/proline/alanine/methionine/isoleucine/valine/histidine/tyrosine/ phenylalanine/cysteine/aspartic acid/lysine/glutamic acid=37.12:12.68:10.05:8. 97:5.99:4.89:4.83:4.25: 4.05:2.78:1.86:1.10:0.73:0.70. Kinetic analysis showed comparable apparent cell-killing rate coefficients (K app) to be 4.03 x 10 3 and 2.92 x 10 3 cells mg -1 h -1 respectively, by SP and SC, evidencing the complementary anti-DU-145 bioactivity in nature.

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