Anticancer activity of rhamnoallosan against DU-145 cells is kinetically complementary to coexisting polyphenolics in psidium guajava budding leaves

Kuan Chou Chen, Chiu Lan Hsieh, Kuan Dar Huang, Yaw Bee Ker, Charng Cherng Chyau, Robert Y. Peng

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Psldlum guajava L. is a valuable farm fruit plant having many medicinal uses. Previously its budding leaves (PE) were shown to contain huge amounts of soluble polyphenols (SP) including (in mg/g) gallic acid (348), catechin (102), epicatechin (60), rutin (100), quercetin (102), and rutin (100) and to exhibit potent anticancer activity. However, reconstitution of these polyphenols recovered only 40% of the original bioactivity, and the soluble carbohydrate (SC) portion in PE was suspected to contribute the remaining. PE contained a novel rhamnoallosan, which had a carbohydrate/protein (w/w) ratio = 29.06%/10.27% (=2.83, average molecular mass of 5029 kDa), characteristically evidencing a peptidoglycan, consisting of a composition (mole % ratio) of rhamnose/allose/arabinose/tallose/ xylose/fucose/glucose/mannose/galactose = 36.05:24.24:8.76:7.95:7.37:5.90:3.69:3.19:2.85 and of amino acid (in wt %) glycine/leucine/proline/alanine/methionine/isoleucine/valine/histidine/tyrosine/ phenylalanine/cysteine/aspartic acid/lysine/glutamic acid=37.12:12.68:10.05:8. 97:5.99:4.89:4.83:4.25: 4.05:2.78:1.86:1.10:0.73:0.70. Kinetic analysis showed comparable apparent cell-killing rate coefficients (K app) to be 4.03 x 10 3 and 2.92 x 10 3 cells mg -1 h -1 respectively, by SP and SC, evidencing the complementary anti-DU-145 bioactivity in nature.

Original languageEnglish
Pages (from-to)6114-6122
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume57
Issue number14
DOIs
Publication statusPublished - 2009 Jul 22

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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