Abstract
Complex transcriptional profile of glutathione S-transferase Delta cluster genes occurred in the developmental process of the fruit fly Drosophila melanogaster. The purpose of this project was to quantify the expression levels of Gst Delta class genes altered by aniline exposure and to understand the relationship between aniline dosages and the variation of Gst Delta genes expressed in D. melanogaster. Using RT-PCR expression assays, the expression patterns of the transcript mRNAs of the glutathione S-transferase Delta genes were revealed and their expression levels were measured at eggs, larvae, pupae and adults. The adult stage was selected for further dose-response assays. After analysis, the results indicated that three Gst Delta genes (Gst D2, Gst D5 and Gst D6) were found to show a peak of up-regulated transcriptional response at 6-8. h of exposure of aniline. Furthermore, the dose-response relationship of their induction levels within the dose regiments (from 1.2 to 2.0. μl/tube) had been measured. The expression patterns and annotations of these genes were discussed in the context.
| Original language | English |
|---|---|
| Pages (from-to) | 622-627 |
| Number of pages | 6 |
| Journal | Environmental Toxicology and Pharmacology |
| Volume | 39 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2015 Mar 1 |
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All Science Journal Classification (ASJC) codes
- Toxicology
- Pharmacology
- Health, Toxicology and Mutagenesis
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Aniline exposure associated with up-regulated transcriptional responses of three glutathione S-transferase Delta genes in Drosophila melanogaster. / Chan, Wen Chiao; Chien, Yi-Chih; Chien, Cheng I.
In: Environmental Toxicology and Pharmacology, Vol. 39, No. 2, 01.03.2015, p. 622-627.Research output: Contribution to journal › Article
TY - JOUR
T1 - Aniline exposure associated with up-regulated transcriptional responses of three glutathione S-transferase Delta genes in Drosophila melanogaster
AU - Chan, Wen Chiao
AU - Chien, Yi-Chih
AU - Chien, Cheng I.
PY - 2015/3/1
Y1 - 2015/3/1
N2 - Complex transcriptional profile of glutathione S-transferase Delta cluster genes occurred in the developmental process of the fruit fly Drosophila melanogaster. The purpose of this project was to quantify the expression levels of Gst Delta class genes altered by aniline exposure and to understand the relationship between aniline dosages and the variation of Gst Delta genes expressed in D. melanogaster. Using RT-PCR expression assays, the expression patterns of the transcript mRNAs of the glutathione S-transferase Delta genes were revealed and their expression levels were measured at eggs, larvae, pupae and adults. The adult stage was selected for further dose-response assays. After analysis, the results indicated that three Gst Delta genes (Gst D2, Gst D5 and Gst D6) were found to show a peak of up-regulated transcriptional response at 6-8. h of exposure of aniline. Furthermore, the dose-response relationship of their induction levels within the dose regiments (from 1.2 to 2.0. μl/tube) had been measured. The expression patterns and annotations of these genes were discussed in the context.
AB - Complex transcriptional profile of glutathione S-transferase Delta cluster genes occurred in the developmental process of the fruit fly Drosophila melanogaster. The purpose of this project was to quantify the expression levels of Gst Delta class genes altered by aniline exposure and to understand the relationship between aniline dosages and the variation of Gst Delta genes expressed in D. melanogaster. Using RT-PCR expression assays, the expression patterns of the transcript mRNAs of the glutathione S-transferase Delta genes were revealed and their expression levels were measured at eggs, larvae, pupae and adults. The adult stage was selected for further dose-response assays. After analysis, the results indicated that three Gst Delta genes (Gst D2, Gst D5 and Gst D6) were found to show a peak of up-regulated transcriptional response at 6-8. h of exposure of aniline. Furthermore, the dose-response relationship of their induction levels within the dose regiments (from 1.2 to 2.0. μl/tube) had been measured. The expression patterns and annotations of these genes were discussed in the context.
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UR - http://www.scopus.com/inward/citedby.url?scp=84922604534&partnerID=8YFLogxK
U2 - 10.1016/j.etap.2014.12.023
DO - 10.1016/j.etap.2014.12.023
M3 - Article
C2 - 25682008
AN - SCOPUS:84922604534
VL - 39
SP - 622
EP - 627
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
SN - 1382-6689
IS - 2
ER -