Analysis of double-stranded DNA by microchip capillary electrophoresis using polymer solutions containing gold nanoparticles

Yang Wei Lin, May Jen Huang, Huan Tsung Chang

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The impact of gold nanoparticles (GNPs) on the microchip electrophoretic separation of double-stranded (ds) DNA using poly(ethylene oxide) (PEO) is described. Coating of the 75-μm separation channel on a poly(methyl methacrylate) (PMMA) plate in sequence with poly(vinyl pyrrolidone), PEO, and 13-nm GNPs is effective to improve reproducibility and resolution. In this study, we have also found that adding 13-nm GNPs to 1.5% PEO is extremely important to achieve high resolution and reproducibility for DNA separation. In terms of the stability of the GNPs, 100 mM glycine-citrate buffer at pH 9.2 is a good buffer system for preparing 1.5% PEO. The separation of DNA markers V and VI ranging in size from 8 to 2176 base pairs has been demonstrated using the three-layer-coated PMMA microdevice filled with 1.5% PEO containing the GNPs. Using these conditions, the analysis of the polymerase chain reaction products of UGT1A7 was complete in 7 min, with the relative standard deviation values of the peak heights and migration times less than 2.3% and 2.0%, respectively. In conjunction with stepwise changes of the concentrations of ethidium bromide (0.5 and 5 μg/ml), this method allows improved resolution and sensitivity for DNA markers V and VI.

Original languageEnglish
Pages (from-to)47-55
Number of pages9
JournalJournal of Chromatography A
Volume1014
Issue number1-2
DOIs
Publication statusPublished - 2003 Oct 3

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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