Analysis of an SMN gene-like DNA fragment.

Y. J. Jong, H. Li, J. G. Chang, H. M. Hsieh-Li, H. H. Lee, C. C. Lee, N. M. Wang, C. H. Tsai

Research output: Contribution to journalArticle

Abstract

Part of a survival motor neuron (SMN) gene-like DNA fragment has been identified. This DNA fragment was accidentally isolated from cDNA by RT-PCR using primers specific for the region between exon 3 and 6 of the SMN gene. This fragment was used as a probe to hybridize the mRNA from several tissues, but we have been unable to detect any transcript of this SMN-like gene in these tissues. Thus, we have inferred this SMN gene-like fragment was a genomic product contaminant that was amplified in the reaction. Sequencing analysis of this fragment, which contains several stop codons, revealed a 74.6% nucleotide homology with the SMN gene. From these results, we believe that this DNA fragment is not a mutated form of SMN gene. Rather, it is an SMN-like pseudogene, which is variably present even in normal individuals.

Original languageEnglish
Pages (from-to)112-116
Number of pages5
JournalThe Kaohsiung journal of medical sciences
Volume16
Issue number3
Publication statusPublished - 2000 Mar

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Motor Neurons
Survival Analysis
DNA
Genes
Pseudogenes
Terminator Codon
Exons
Nucleotides
Complementary DNA
Polymerase Chain Reaction
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Jong, Y. J., Li, H., Chang, J. G., Hsieh-Li, H. M., Lee, H. H., Lee, C. C., ... Tsai, C. H. (2000). Analysis of an SMN gene-like DNA fragment. The Kaohsiung journal of medical sciences, 16(3), 112-116.
Jong, Y. J. ; Li, H. ; Chang, J. G. ; Hsieh-Li, H. M. ; Lee, H. H. ; Lee, C. C. ; Wang, N. M. ; Tsai, C. H. / Analysis of an SMN gene-like DNA fragment. In: The Kaohsiung journal of medical sciences. 2000 ; Vol. 16, No. 3. pp. 112-116.
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Jong, YJ, Li, H, Chang, JG, Hsieh-Li, HM, Lee, HH, Lee, CC, Wang, NM & Tsai, CH 2000, 'Analysis of an SMN gene-like DNA fragment.', The Kaohsiung journal of medical sciences, vol. 16, no. 3, pp. 112-116.

Analysis of an SMN gene-like DNA fragment. / Jong, Y. J.; Li, H.; Chang, J. G.; Hsieh-Li, H. M.; Lee, H. H.; Lee, C. C.; Wang, N. M.; Tsai, C. H.

In: The Kaohsiung journal of medical sciences, Vol. 16, No. 3, 03.2000, p. 112-116.

Research output: Contribution to journalArticle

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Jong YJ, Li H, Chang JG, Hsieh-Li HM, Lee HH, Lee CC et al. Analysis of an SMN gene-like DNA fragment. The Kaohsiung journal of medical sciences. 2000 Mar;16(3):112-116.