Analysis of an SMN gene-like DNA fragment.

Y. J. Jong, H. Li, J. G. Chang, H. M. Hsieh-Li, H. H. Lee, C. C. Lee, N. M. Wang, C. H. Tsai

Research output: Contribution to journalArticle

Abstract

Part of a survival motor neuron (SMN) gene-like DNA fragment has been identified. This DNA fragment was accidentally isolated from cDNA by RT-PCR using primers specific for the region between exon 3 and 6 of the SMN gene. This fragment was used as a probe to hybridize the mRNA from several tissues, but we have been unable to detect any transcript of this SMN-like gene in these tissues. Thus, we have inferred this SMN gene-like fragment was a genomic product contaminant that was amplified in the reaction. Sequencing analysis of this fragment, which contains several stop codons, revealed a 74.6% nucleotide homology with the SMN gene. From these results, we believe that this DNA fragment is not a mutated form of SMN gene. Rather, it is an SMN-like pseudogene, which is variably present even in normal individuals.

Original languageEnglish
Pages (from-to)112-116
Number of pages5
JournalThe Kaohsiung journal of medical sciences
Volume16
Issue number3
Publication statusPublished - 2000 Mar

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Jong, Y. J., Li, H., Chang, J. G., Hsieh-Li, H. M., Lee, H. H., Lee, C. C., Wang, N. M., & Tsai, C. H. (2000). Analysis of an SMN gene-like DNA fragment. The Kaohsiung journal of medical sciences, 16(3), 112-116.