Aloe-emodin is an interferon-inducing agent with antiviral activity against Japanese encephalitis virus and enterovirus 71

Cheng Wen Lin, Chia Fang Wu, Nai-Wan Hsiao, Ching Yao Chang, Shih Wein Li, Lei Wan, Ying Ju Lin, Wei Yong Lin

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

In this study, aloe-emodin was identified as a potential interferon (IFN)-inducer by screening compounds from Chinese herbal medicine. Aloe-emodin showed low cytotoxicity to human HL-CZ promonocyte cells and TE-671 medulloblastoma cells and significantly activated interferon-stimulated response element (ISRE) and gamma-activated sequence (GAS)-driven cis-reporting systems. Moreover, aloe-emodin upregulated expression of IFN-stimulated genes such as dsRNA-activated protein kinase and 2′,5′-oligoisoadenylate synthase. Aloe-emodin resulted in significant activation of nitric oxide production. The antiviral activity of aloe-emodin against Japanese encephalitis virus (JEV) and enterovirus 71 (EV71) was evaluated using dose- and time-dependent plaque reduction assays in HL-CZ cells and TE-671 cells. The 50% inhibitory concentration (IC50) of aloe-emodin ranged from 0.50 μg/mL to 1.51 μg/mL for JEV and from 0.14 μg/mL to 0.52 μg/mL for EV71. Aloe-emodin showed clearly potent virus inhibitory abilities and achieved high therapeutic indices, in particular for HL-CZ cells. Therefore, the study demonstrated dose- and time-dependent actions of aloe-emodin on the inhibition of JEV and EV71 replication via IFN signalling responses.

Original languageEnglish
Pages (from-to)355-359
Number of pages5
JournalInternational Journal of Antimicrobial Agents
Volume32
Issue number4
DOIs
Publication statusPublished - 2008 Oct 1

Fingerprint

Japanese Encephalitis Virus
Enterovirus
Interferons
Antiviral Agents
Inhibitory Concentration 50
Monocyte-Macrophage Precursor Cells
Interferon Inducers
Medulloblastoma
aloe emodin
Herbal Medicine
Response Elements
Protein Kinases
Nitric Oxide

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

Cite this

Lin, Cheng Wen ; Wu, Chia Fang ; Hsiao, Nai-Wan ; Chang, Ching Yao ; Li, Shih Wein ; Wan, Lei ; Lin, Ying Ju ; Lin, Wei Yong. / Aloe-emodin is an interferon-inducing agent with antiviral activity against Japanese encephalitis virus and enterovirus 71. In: International Journal of Antimicrobial Agents. 2008 ; Vol. 32, No. 4. pp. 355-359.
@article{273dba1b5cc24cb5a219626f80198e55,
title = "Aloe-emodin is an interferon-inducing agent with antiviral activity against Japanese encephalitis virus and enterovirus 71",
abstract = "In this study, aloe-emodin was identified as a potential interferon (IFN)-inducer by screening compounds from Chinese herbal medicine. Aloe-emodin showed low cytotoxicity to human HL-CZ promonocyte cells and TE-671 medulloblastoma cells and significantly activated interferon-stimulated response element (ISRE) and gamma-activated sequence (GAS)-driven cis-reporting systems. Moreover, aloe-emodin upregulated expression of IFN-stimulated genes such as dsRNA-activated protein kinase and 2′,5′-oligoisoadenylate synthase. Aloe-emodin resulted in significant activation of nitric oxide production. The antiviral activity of aloe-emodin against Japanese encephalitis virus (JEV) and enterovirus 71 (EV71) was evaluated using dose- and time-dependent plaque reduction assays in HL-CZ cells and TE-671 cells. The 50{\%} inhibitory concentration (IC50) of aloe-emodin ranged from 0.50 μg/mL to 1.51 μg/mL for JEV and from 0.14 μg/mL to 0.52 μg/mL for EV71. Aloe-emodin showed clearly potent virus inhibitory abilities and achieved high therapeutic indices, in particular for HL-CZ cells. Therefore, the study demonstrated dose- and time-dependent actions of aloe-emodin on the inhibition of JEV and EV71 replication via IFN signalling responses.",
author = "Lin, {Cheng Wen} and Wu, {Chia Fang} and Nai-Wan Hsiao and Chang, {Ching Yao} and Li, {Shih Wein} and Lei Wan and Lin, {Ying Ju} and Lin, {Wei Yong}",
year = "2008",
month = "10",
day = "1",
doi = "10.1016/j.ijantimicag.2008.04.018",
language = "English",
volume = "32",
pages = "355--359",
journal = "International Journal of Antimicrobial Agents",
issn = "0924-8579",
publisher = "Elsevier",
number = "4",

}

Aloe-emodin is an interferon-inducing agent with antiviral activity against Japanese encephalitis virus and enterovirus 71. / Lin, Cheng Wen; Wu, Chia Fang; Hsiao, Nai-Wan; Chang, Ching Yao; Li, Shih Wein; Wan, Lei; Lin, Ying Ju; Lin, Wei Yong.

In: International Journal of Antimicrobial Agents, Vol. 32, No. 4, 01.10.2008, p. 355-359.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Aloe-emodin is an interferon-inducing agent with antiviral activity against Japanese encephalitis virus and enterovirus 71

AU - Lin, Cheng Wen

AU - Wu, Chia Fang

AU - Hsiao, Nai-Wan

AU - Chang, Ching Yao

AU - Li, Shih Wein

AU - Wan, Lei

AU - Lin, Ying Ju

AU - Lin, Wei Yong

PY - 2008/10/1

Y1 - 2008/10/1

N2 - In this study, aloe-emodin was identified as a potential interferon (IFN)-inducer by screening compounds from Chinese herbal medicine. Aloe-emodin showed low cytotoxicity to human HL-CZ promonocyte cells and TE-671 medulloblastoma cells and significantly activated interferon-stimulated response element (ISRE) and gamma-activated sequence (GAS)-driven cis-reporting systems. Moreover, aloe-emodin upregulated expression of IFN-stimulated genes such as dsRNA-activated protein kinase and 2′,5′-oligoisoadenylate synthase. Aloe-emodin resulted in significant activation of nitric oxide production. The antiviral activity of aloe-emodin against Japanese encephalitis virus (JEV) and enterovirus 71 (EV71) was evaluated using dose- and time-dependent plaque reduction assays in HL-CZ cells and TE-671 cells. The 50% inhibitory concentration (IC50) of aloe-emodin ranged from 0.50 μg/mL to 1.51 μg/mL for JEV and from 0.14 μg/mL to 0.52 μg/mL for EV71. Aloe-emodin showed clearly potent virus inhibitory abilities and achieved high therapeutic indices, in particular for HL-CZ cells. Therefore, the study demonstrated dose- and time-dependent actions of aloe-emodin on the inhibition of JEV and EV71 replication via IFN signalling responses.

AB - In this study, aloe-emodin was identified as a potential interferon (IFN)-inducer by screening compounds from Chinese herbal medicine. Aloe-emodin showed low cytotoxicity to human HL-CZ promonocyte cells and TE-671 medulloblastoma cells and significantly activated interferon-stimulated response element (ISRE) and gamma-activated sequence (GAS)-driven cis-reporting systems. Moreover, aloe-emodin upregulated expression of IFN-stimulated genes such as dsRNA-activated protein kinase and 2′,5′-oligoisoadenylate synthase. Aloe-emodin resulted in significant activation of nitric oxide production. The antiviral activity of aloe-emodin against Japanese encephalitis virus (JEV) and enterovirus 71 (EV71) was evaluated using dose- and time-dependent plaque reduction assays in HL-CZ cells and TE-671 cells. The 50% inhibitory concentration (IC50) of aloe-emodin ranged from 0.50 μg/mL to 1.51 μg/mL for JEV and from 0.14 μg/mL to 0.52 μg/mL for EV71. Aloe-emodin showed clearly potent virus inhibitory abilities and achieved high therapeutic indices, in particular for HL-CZ cells. Therefore, the study demonstrated dose- and time-dependent actions of aloe-emodin on the inhibition of JEV and EV71 replication via IFN signalling responses.

UR - http://www.scopus.com/inward/record.url?scp=50549094449&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=50549094449&partnerID=8YFLogxK

U2 - 10.1016/j.ijantimicag.2008.04.018

DO - 10.1016/j.ijantimicag.2008.04.018

M3 - Article

C2 - 18701259

AN - SCOPUS:50549094449

VL - 32

SP - 355

EP - 359

JO - International Journal of Antimicrobial Agents

JF - International Journal of Antimicrobial Agents

SN - 0924-8579

IS - 4

ER -