Abstract
We developed a method for the determination of three aminothiols-cysteine, glutathione (GSH), and homocysteine-using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). The analytes were first captured using the unmodified 14-nm gold nanoparticles; N-2-mercaptopropionylglycine-modified gold nanoparticles serving as internal standard were sequentially added, and then the sample was analyzed using SALDI-MS. This approach provided good quantitative linearity of the three analytes (R2 = ~0.99), with good reproducibility (relative standard deviations: <10%), in the analyses of GSH in the lysates of human red blood cells and MCF-7 cancer breast cells in the presence and absence of the anti-inflammatory drug sulfasalazine. The internal-standard SALDI-MS approach provides simplicity, accuracy, and precision to the determination of GSH in cells under drug invasion, to open an avenue for SALDI-MS to be used for the precise quantitative determination of a variety of analytes. From the Clinical Editor: This paper reports the development of a surface assisted laser desorption/ionization mass spectrometry method to precisely determine aminothiols-cysteine (Cys), glutathione (GSH), and homocysteine (HCys).
| Original language | English |
|---|---|
| Pages (from-to) | 530-537 |
| Number of pages | 8 |
| Journal | Nanomedicine: Nanotechnology, Biology, and Medicine |
| Volume | 6 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2010 Aug 1 |
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All Science Journal Classification (ASJC) codes
- Medicine (miscellaneous)
- Bioengineering
- Biomedical Engineering
- Molecular Medicine
- Materials Science(all)
- Pharmaceutical Science
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Accurate quantitation of glutathione in cell lysates through surface-assisted laser desorption/ionization mass spectrometry using gold nanoparticles. / Chiang, Cheng Kang; Lin, Yang-Wei; Chen, Wen Tsen; Chang, Huan Tsung.
In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 6, No. 4, 01.08.2010, p. 530-537.Research output: Contribution to journal › Article
TY - JOUR
T1 - Accurate quantitation of glutathione in cell lysates through surface-assisted laser desorption/ionization mass spectrometry using gold nanoparticles
AU - Chiang, Cheng Kang
AU - Lin, Yang-Wei
AU - Chen, Wen Tsen
AU - Chang, Huan Tsung
PY - 2010/8/1
Y1 - 2010/8/1
N2 - We developed a method for the determination of three aminothiols-cysteine, glutathione (GSH), and homocysteine-using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). The analytes were first captured using the unmodified 14-nm gold nanoparticles; N-2-mercaptopropionylglycine-modified gold nanoparticles serving as internal standard were sequentially added, and then the sample was analyzed using SALDI-MS. This approach provided good quantitative linearity of the three analytes (R2 = ~0.99), with good reproducibility (relative standard deviations: <10%), in the analyses of GSH in the lysates of human red blood cells and MCF-7 cancer breast cells in the presence and absence of the anti-inflammatory drug sulfasalazine. The internal-standard SALDI-MS approach provides simplicity, accuracy, and precision to the determination of GSH in cells under drug invasion, to open an avenue for SALDI-MS to be used for the precise quantitative determination of a variety of analytes. From the Clinical Editor: This paper reports the development of a surface assisted laser desorption/ionization mass spectrometry method to precisely determine aminothiols-cysteine (Cys), glutathione (GSH), and homocysteine (HCys).
AB - We developed a method for the determination of three aminothiols-cysteine, glutathione (GSH), and homocysteine-using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). The analytes were first captured using the unmodified 14-nm gold nanoparticles; N-2-mercaptopropionylglycine-modified gold nanoparticles serving as internal standard were sequentially added, and then the sample was analyzed using SALDI-MS. This approach provided good quantitative linearity of the three analytes (R2 = ~0.99), with good reproducibility (relative standard deviations: <10%), in the analyses of GSH in the lysates of human red blood cells and MCF-7 cancer breast cells in the presence and absence of the anti-inflammatory drug sulfasalazine. The internal-standard SALDI-MS approach provides simplicity, accuracy, and precision to the determination of GSH in cells under drug invasion, to open an avenue for SALDI-MS to be used for the precise quantitative determination of a variety of analytes. From the Clinical Editor: This paper reports the development of a surface assisted laser desorption/ionization mass spectrometry method to precisely determine aminothiols-cysteine (Cys), glutathione (GSH), and homocysteine (HCys).
UR - http://www.scopus.com/inward/record.url?scp=77955277990&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77955277990&partnerID=8YFLogxK
U2 - 10.1016/j.nano.2010.01.006
DO - 10.1016/j.nano.2010.01.006
M3 - Article
VL - 6
SP - 530
EP - 537
JO - Nanomedicine: Nanotechnology, Biology, and Medicine
JF - Nanomedicine: Nanotechnology, Biology, and Medicine
SN - 1549-9634
IS - 4
ER -