Accurate quantitation of glutathione in cell lysates through surface-assisted laser desorption/ionization mass spectrometry using gold nanoparticles

Cheng Kang Chiang, Yang-Wei Lin, Wen Tsen Chen, Huan Tsung Chang

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

We developed a method for the determination of three aminothiols-cysteine, glutathione (GSH), and homocysteine-using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). The analytes were first captured using the unmodified 14-nm gold nanoparticles; N-2-mercaptopropionylglycine-modified gold nanoparticles serving as internal standard were sequentially added, and then the sample was analyzed using SALDI-MS. This approach provided good quantitative linearity of the three analytes (R2 = ~0.99), with good reproducibility (relative standard deviations: <10%), in the analyses of GSH in the lysates of human red blood cells and MCF-7 cancer breast cells in the presence and absence of the anti-inflammatory drug sulfasalazine. The internal-standard SALDI-MS approach provides simplicity, accuracy, and precision to the determination of GSH in cells under drug invasion, to open an avenue for SALDI-MS to be used for the precise quantitative determination of a variety of analytes. From the Clinical Editor: This paper reports the development of a surface assisted laser desorption/ionization mass spectrometry method to precisely determine aminothiols-cysteine (Cys), glutathione (GSH), and homocysteine (HCys).

Original languageEnglish
Pages (from-to)530-537
Number of pages8
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume6
Issue number4
DOIs
Publication statusPublished - 2010 Aug 1

Fingerprint

Gold
Nanoparticles
Ionization
Mass spectrometry
Glutathione
Desorption
Mass Spectrometry
Lasers
Homocysteine
Cysteine
Tiopronin
Sulfasalazine
Pharmaceutical Preparations
Blood
Anti-Inflammatory Agents
Erythrocytes
Cells
Breast Neoplasms

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Bioengineering
  • Biomedical Engineering
  • Molecular Medicine
  • Materials Science(all)
  • Pharmaceutical Science

Cite this

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abstract = "We developed a method for the determination of three aminothiols-cysteine, glutathione (GSH), and homocysteine-using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). The analytes were first captured using the unmodified 14-nm gold nanoparticles; N-2-mercaptopropionylglycine-modified gold nanoparticles serving as internal standard were sequentially added, and then the sample was analyzed using SALDI-MS. This approach provided good quantitative linearity of the three analytes (R2 = ~0.99), with good reproducibility (relative standard deviations: <10{\%}), in the analyses of GSH in the lysates of human red blood cells and MCF-7 cancer breast cells in the presence and absence of the anti-inflammatory drug sulfasalazine. The internal-standard SALDI-MS approach provides simplicity, accuracy, and precision to the determination of GSH in cells under drug invasion, to open an avenue for SALDI-MS to be used for the precise quantitative determination of a variety of analytes. From the Clinical Editor: This paper reports the development of a surface assisted laser desorption/ionization mass spectrometry method to precisely determine aminothiols-cysteine (Cys), glutathione (GSH), and homocysteine (HCys).",
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Accurate quantitation of glutathione in cell lysates through surface-assisted laser desorption/ionization mass spectrometry using gold nanoparticles. / Chiang, Cheng Kang; Lin, Yang-Wei; Chen, Wen Tsen; Chang, Huan Tsung.

In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 6, No. 4, 01.08.2010, p. 530-537.

Research output: Contribution to journalArticle

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